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ecl enhanced chemiluminescence detection kit  (Cytiva Europe)


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    Cytiva Europe ecl enhanced chemiluminescence detection kit
    Ecl Enhanced Chemiluminescence Detection Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+detection+kit/Amersham+ECL+Western+blot+detection+kit/pm42790027-56-5-10
    Average 94 stars, based on 214 article reviews
    ecl enhanced chemiluminescence detection kit - by Bioz Stars, 2026-10
    94/100 stars

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    other:

    Article Title: Methods and compositions for treating cell senescence accumulation related disease
    Article Snippet: Protein concentrations were determined using the BCA Protein Assay Kit (Interchim).

    Nucleic Acid Electrophoresis:

    Article Title: Mex-3 RNA Binding Family Member A Modulates Peroxisome Proliferator-Activated Receptor Gamma Pathway Activity and Colorectal Cancer Growth.
    Article Snippet: Cells were lysed on ice for 30 minutes with lysis buffer containing 20mM Tris-HCl (pH, 7.5), 150 mM NaCl, 2 mM EDTA, 0.1% (v/v) sodium deoxycholate, and 0.1% (v/v) sodium dodecyl sulfate supplemented with 1× Complete protease inhibitor cocktail (Roche Applied Science), 20 mM NaF, 1 mM PMSF, and 1mM Na3VO4. .. Twenty to 50 μg of each protein extract were run in a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, transferred to a nitrocellulose membrane, and incubated overnight with the desired antibodies (Table 3) after which signals were revealed with ECL detection kit (GE Healthcare Life Sciences). .. Protein concentration was determined with BCA Protein Assay kit (Thermo Fisher Scientific).

    Membrane:

    Article Title: Mex-3 RNA Binding Family Member A Modulates Peroxisome Proliferator-Activated Receptor Gamma Pathway Activity and Colorectal Cancer Growth.
    Article Snippet: Cells were lysed on ice for 30 minutes with lysis buffer containing 20mM Tris-HCl (pH, 7.5), 150 mM NaCl, 2 mM EDTA, 0.1% (v/v) sodium deoxycholate, and 0.1% (v/v) sodium dodecyl sulfate supplemented with 1× Complete protease inhibitor cocktail (Roche Applied Science), 20 mM NaF, 1 mM PMSF, and 1mM Na3VO4. .. Twenty to 50 μg of each protein extract were run in a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, transferred to a nitrocellulose membrane, and incubated overnight with the desired antibodies (Table 3) after which signals were revealed with ECL detection kit (GE Healthcare Life Sciences). .. Protein concentration was determined with BCA Protein Assay kit (Thermo Fisher Scientific).

    Incubation:

    Article Title: Mex-3 RNA Binding Family Member A Modulates Peroxisome Proliferator-Activated Receptor Gamma Pathway Activity and Colorectal Cancer Growth.
    Article Snippet: Cells were lysed on ice for 30 minutes with lysis buffer containing 20mM Tris-HCl (pH, 7.5), 150 mM NaCl, 2 mM EDTA, 0.1% (v/v) sodium deoxycholate, and 0.1% (v/v) sodium dodecyl sulfate supplemented with 1× Complete protease inhibitor cocktail (Roche Applied Science), 20 mM NaF, 1 mM PMSF, and 1mM Na3VO4. .. Twenty to 50 μg of each protein extract were run in a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, transferred to a nitrocellulose membrane, and incubated overnight with the desired antibodies (Table 3) after which signals were revealed with ECL detection kit (GE Healthcare Life Sciences). .. Protein concentration was determined with BCA Protein Assay kit (Thermo Fisher Scientific).

    Article Title: Characterization of a SARS-CoV-2 infection model in golden hamsters with diabetes mellitus
    Article Snippet: Anti-GAPDH mouse monoclonal antibodies (Proteintech, 1:10,000) and anti-Leptein receptor rabbit polyclonal antibodies (HUABIO, 1:2000) were used as primary antibodies and incubated with the membranes at 4 °C for 12 h. .. After washing twice with PBS containing 0.2% Tween-20 (PBST), the membranes were incubated with HRP-linked goat anti-rabbit antibodies (ThermoFisher Scientific, 31,466, 1:5000) or HRP-linked anti-mouse IgG (ThermoFisher Scientific, 31,430, 1:5000) at room temperature for 1 h before visualization using the ECL Detection kit (GE Healthcare). ..

    Article Title: Isolation and long‐term culture of primary mouse cholangiocytes that retain biophysical properties and distinct Cl‐conductances: An initial study
    Article Snippet: .. This was followed by incubation with peroxidase‐conjugated goat anti‐rabbit antibody (1:10,000 dilution, Jackson Immunoresearch Laboratories, Inc., catalog no. 111‐035‐003) and visualized by the ECL + detection kit (GE Healthcare, England, catalog no. RPN2209). .. Primary antibodies used were CK19 (10712‐1‐AP, Proteintech, 1:500), CK7 (SC‐23876, Santa Cruz Biotechnology, Inc. Dallas, TX, 1:500), CFTR (Ab‐737) (8B0860, Syd labs, Hopkinton, MA, 1:200), and TMEM16A/ANO1(H‐41) (SC‐135235, Santa Cruz, 1:500).



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